recombinant murine stem cell factor (R&D Systems)
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recombinant murine stem cell factor
Recombinant Murine Stem Cell Factor, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+murine+stem+cell+factor/Recombinant+Mouse+SCF+Protein/pm39607037-231-118-123
Average 95 stars, based on 145 article reviews
Recombinant Murine Stem Cell Factor, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+murine+stem+cell+factor/Recombinant+Mouse+SCF+Protein/pm39607037-231-118-123
Average 95 stars, based on 145 article reviews
recombinant murine stem cell factor - by Bioz Stars,
2026-09
95/100 stars
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Transgenic Assay:Article Title: Heavy chain-only antibodies with a stabilized human VH in transgenic chickens for therapeutic antibody discovery. Article Snippet: .. For insertion into the genome of chicken primordial germ cells and production of transgenic birds, a β-actin promoter and attB site were included on the vector for integration by phiC31 integrase into an attP site previously targeted to the chicken heavy-chain locus.34,35 A loxP site was also included for removal of all selectable markers and plasmid backbone elements by Cre recombination.36 Chicken primordial germ cells (PGCs) were derived and cultured as described,36–38 in KO-DMEM (ThermoFisher 0,829,018) containing 40% buffalo rat liver (BRL)conditioned medium, 7.5% fetal calf serum (FCS) (Hyclone, SH30088.03), 2.5% chicken serum (ThermoFisher 16,110,082), 2 mm Glutamax (ThermoFisher 35,050–061), 1 mm pyruvate (ThermoFisher 11,360-070), 1X non-essential amino acids (ThermoFisher 11,140–050), 0.1 mm β-mercapto-ethanol (ThermoFisher 21,985–023), 6 ng/ml Plasmid Preparation:Article Title: Heavy chain-only antibodies with a stabilized human VH in transgenic chickens for therapeutic antibody discovery. Article Snippet: .. For insertion into the genome of chicken primordial germ cells and production of transgenic birds, a β-actin promoter and attB site were included on the vector for integration by phiC31 integrase into an attP site previously targeted to the chicken heavy-chain locus.34,35 A loxP site was also included for removal of all selectable markers and plasmid backbone elements by Cre recombination.36 Chicken primordial germ cells (PGCs) were derived and cultured as described,36–38 in KO-DMEM (ThermoFisher 0,829,018) containing 40% buffalo rat liver (BRL)conditioned medium, 7.5% fetal calf serum (FCS) (Hyclone, SH30088.03), 2.5% chicken serum (ThermoFisher 16,110,082), 2 mm Glutamax (ThermoFisher 35,050–061), 1 mm pyruvate (ThermoFisher 11,360-070), 1X non-essential amino acids (ThermoFisher 11,140–050), 0.1 mm β-mercapto-ethanol (ThermoFisher 21,985–023), 6 ng/ml Derivative Assay:Article Title: Heavy chain-only antibodies with a stabilized human VH in transgenic chickens for therapeutic antibody discovery. Article Snippet: .. For insertion into the genome of chicken primordial germ cells and production of transgenic birds, a β-actin promoter and attB site were included on the vector for integration by phiC31 integrase into an attP site previously targeted to the chicken heavy-chain locus.34,35 A loxP site was also included for removal of all selectable markers and plasmid backbone elements by Cre recombination.36 Chicken primordial germ cells (PGCs) were derived and cultured as described,36–38 in KO-DMEM (ThermoFisher 0,829,018) containing 40% buffalo rat liver (BRL)conditioned medium, 7.5% fetal calf serum (FCS) (Hyclone, SH30088.03), 2.5% chicken serum (ThermoFisher 16,110,082), 2 mm Glutamax (ThermoFisher 35,050–061), 1 mm pyruvate (ThermoFisher 11,360-070), 1X non-essential amino acids (ThermoFisher 11,140–050), 0.1 mm β-mercapto-ethanol (ThermoFisher 21,985–023), 6 ng/ml Article Title: Heavy chain-only antibodies with a stabilized human VH in transgenic chickens for therapeutic antibody discovery Article Snippet: .. Chicken primordial germ cells (PGCs) were derived and cultured as described, in KO-DMEM (ThermoFisher 0,829,018) containing 40% buffalo rat liver (BRL)-conditioned medium, 7.5% fetal calf serum (FCS) (Hyclone, SH30088.03), 2.5% chicken serum (ThermoFisher 16,110,082), 2 mm Glutamax (ThermoFisher 35,050–061), 1 mm pyruvate (ThermoFisher 11,360-070), 1X non-essential amino acids (ThermoFisher 11,140–050), 0.1 mm β-mercapto-ethanol (ThermoFisher 21,985–023), 6 ng/ml Cell Culture:Article Title: Heavy chain-only antibodies with a stabilized human VH in transgenic chickens for therapeutic antibody discovery. Article Snippet: .. For insertion into the genome of chicken primordial germ cells and production of transgenic birds, a β-actin promoter and attB site were included on the vector for integration by phiC31 integrase into an attP site previously targeted to the chicken heavy-chain locus.34,35 A loxP site was also included for removal of all selectable markers and plasmid backbone elements by Cre recombination.36 Chicken primordial germ cells (PGCs) were derived and cultured as described,36–38 in KO-DMEM (ThermoFisher 0,829,018) containing 40% buffalo rat liver (BRL)conditioned medium, 7.5% fetal calf serum (FCS) (Hyclone, SH30088.03), 2.5% chicken serum (ThermoFisher 16,110,082), 2 mm Glutamax (ThermoFisher 35,050–061), 1 mm pyruvate (ThermoFisher 11,360-070), 1X non-essential amino acids (ThermoFisher 11,140–050), 0.1 mm β-mercapto-ethanol (ThermoFisher 21,985–023), 6 ng/ml Article Title: Chickens with a Truncated Light Chain Transgene Express Single-Domain H Chain–Only Antibodies Article Snippet: .. Chicken primordial germ cells of line 229-92, carrying the attP-neo replacement of the L chain, were cultured as described previously ( 17–20 ) in KO-DMEM (Thermo Fisher, Waltham MA, 10829018) containing 40% Buffalo rat liver (BRL)-conditioned medium, 7.5% FCS (Hyclone, South Logan, UT, SH30088.03), 2.5% chicken serum (Thermo Fisher, 16110082), 2 mM GlutaMAX (Thermo Fisher, 35050-061), 1 mM pyruvate (Thermo Fisher, 11360-070), 1× nonessential amino acids (Thermo Fisher, 11140-050), 0.1 mM 2-ME (Thermo Fisher, 21985-023), 6 ng/ml Article Title: Covalent inhibition of NSD1 histone methyltransferase Article Snippet: .. The NUP98-NSD1 cells were cultured in IMDM medium supplemented with 15% FCS, penicillin-streptomycin and Article Title: Heavy chain-only antibodies with a stabilized human VH in transgenic chickens for therapeutic antibody discovery Article Snippet: .. Chicken primordial germ cells (PGCs) were derived and cultured as described, in KO-DMEM (ThermoFisher 0,829,018) containing 40% buffalo rat liver (BRL)-conditioned medium, 7.5% fetal calf serum (FCS) (Hyclone, SH30088.03), 2.5% chicken serum (ThermoFisher 16,110,082), 2 mm Glutamax (ThermoFisher 35,050–061), 1 mm pyruvate (ThermoFisher 11,360-070), 1X non-essential amino acids (ThermoFisher 11,140–050), 0.1 mm β-mercapto-ethanol (ThermoFisher 21,985–023), 6 ng/ml Article Title: Skin microbiome promotes mast cell maturation by triggering stem cell factor (SCF) production in keratinocytes Article Snippet: .. Briefly, bone marrow cells were cultured in RPMI 1640 medium (Invitrogen) supplemented with recombinant murine IL-3 (1 ng/ml, R&D Systems) and Recombinant:Article Title: Heavy chain-only antibodies with a stabilized human VH in transgenic chickens for therapeutic antibody discovery. Article Snippet: .. For insertion into the genome of chicken primordial germ cells and production of transgenic birds, a β-actin promoter and attB site were included on the vector for integration by phiC31 integrase into an attP site previously targeted to the chicken heavy-chain locus.34,35 A loxP site was also included for removal of all selectable markers and plasmid backbone elements by Cre recombination.36 Chicken primordial germ cells (PGCs) were derived and cultured as described,36–38 in KO-DMEM (ThermoFisher 0,829,018) containing 40% buffalo rat liver (BRL)conditioned medium, 7.5% fetal calf serum (FCS) (Hyclone, SH30088.03), 2.5% chicken serum (ThermoFisher 16,110,082), 2 mm Glutamax (ThermoFisher 35,050–061), 1 mm pyruvate (ThermoFisher 11,360-070), 1X non-essential amino acids (ThermoFisher 11,140–050), 0.1 mm β-mercapto-ethanol (ThermoFisher 21,985–023), 6 ng/ml Article Title: Chickens with a Truncated Light Chain Transgene Express Single-Domain H Chain–Only Antibodies Article Snippet: .. Chicken primordial germ cells of line 229-92, carrying the attP-neo replacement of the L chain, were cultured as described previously ( 17–20 ) in KO-DMEM (Thermo Fisher, Waltham MA, 10829018) containing 40% Buffalo rat liver (BRL)-conditioned medium, 7.5% FCS (Hyclone, South Logan, UT, SH30088.03), 2.5% chicken serum (Thermo Fisher, 16110082), 2 mM GlutaMAX (Thermo Fisher, 35050-061), 1 mM pyruvate (Thermo Fisher, 11360-070), 1× nonessential amino acids (Thermo Fisher, 11140-050), 0.1 mM 2-ME (Thermo Fisher, 21985-023), 6 ng/ml Article Title: Covalent inhibition of NSD1 histone methyltransferase Article Snippet: .. The NUP98-NSD1 cells were cultured in IMDM medium supplemented with 15% FCS, penicillin-streptomycin and Article Title: Tagged IDS causes efficient and engraftment-independent prevention of brain pathology during lentiviral gene therapy for Mucopolysaccharidosis type II Article Snippet: Hematopoietic stem and progenitor cells were enriched by lineage depletion using the Lineage Cell Depletion Kit - mouse (Miltenyi Biotec). .. Lin − cells were seeded at a density of 10 6 cells/mL in StemSpan SF expansion medium (STEMCELL Technologies) supplemented with recombinant murine thrombopoietin (10 ng/mL, R&D Systems), Article Title: Heavy chain-only antibodies with a stabilized human VH in transgenic chickens for therapeutic antibody discovery Article Snippet: .. Chicken primordial germ cells (PGCs) were derived and cultured as described, in KO-DMEM (ThermoFisher 0,829,018) containing 40% buffalo rat liver (BRL)-conditioned medium, 7.5% fetal calf serum (FCS) (Hyclone, SH30088.03), 2.5% chicken serum (ThermoFisher 16,110,082), 2 mm Glutamax (ThermoFisher 35,050–061), 1 mm pyruvate (ThermoFisher 11,360-070), 1X non-essential amino acids (ThermoFisher 11,140–050), 0.1 mm β-mercapto-ethanol (ThermoFisher 21,985–023), 6 ng/ml Article Title: Multiplex genome editing of mammalian cells for producing recombinant heparin. Article Snippet: Primary murine mast cells were generated by extracting bone marrow cells from the femurs of 5–8-week-old female mice and culturing the cells in RPMI 1640 medium (Gibco) supplemented with 10% inactivated FBS (ThermoFisher), 25 mM HEPES (pH 7.4), 4 mM Lglutamine, 0.1 mM nonessential amino acids, 1 mM sodium pyruvate, 50 μM β-mercaptoethanol, 100 IU/mL penicillin and 100 μg/ml streptomycin. .. Recombinant murine IL-3 (1 ng/mL; R&D Systems) and Article Title: Skin microbiome promotes mast cell maturation by triggering stem cell factor (SCF) production in keratinocytes Article Snippet: .. Briefly, bone marrow cells were cultured in RPMI 1640 medium (Invitrogen) supplemented with recombinant murine IL-3 (1 ng/ml, R&D Systems) and Irradiation:Article Title: Heavy chain-only antibodies with a stabilized human VH in transgenic chickens for therapeutic antibody discovery. Article Snippet: .. For insertion into the genome of chicken primordial germ cells and production of transgenic birds, a β-actin promoter and attB site were included on the vector for integration by phiC31 integrase into an attP site previously targeted to the chicken heavy-chain locus.34,35 A loxP site was also included for removal of all selectable markers and plasmid backbone elements by Cre recombination.36 Chicken primordial germ cells (PGCs) were derived and cultured as described,36–38 in KO-DMEM (ThermoFisher 0,829,018) containing 40% buffalo rat liver (BRL)conditioned medium, 7.5% fetal calf serum (FCS) (Hyclone, SH30088.03), 2.5% chicken serum (ThermoFisher 16,110,082), 2 mm Glutamax (ThermoFisher 35,050–061), 1 mm pyruvate (ThermoFisher 11,360-070), 1X non-essential amino acids (ThermoFisher 11,140–050), 0.1 mm β-mercapto-ethanol (ThermoFisher 21,985–023), 6 ng/ml Article Title: Chickens with a Truncated Light Chain Transgene Express Single-Domain H Chain–Only Antibodies Article Snippet: .. Chicken primordial germ cells of line 229-92, carrying the attP-neo replacement of the L chain, were cultured as described previously ( 17–20 ) in KO-DMEM (Thermo Fisher, Waltham MA, 10829018) containing 40% Buffalo rat liver (BRL)-conditioned medium, 7.5% FCS (Hyclone, South Logan, UT, SH30088.03), 2.5% chicken serum (Thermo Fisher, 16110082), 2 mM GlutaMAX (Thermo Fisher, 35050-061), 1 mM pyruvate (Thermo Fisher, 11360-070), 1× nonessential amino acids (Thermo Fisher, 11140-050), 0.1 mM 2-ME (Thermo Fisher, 21985-023), 6 ng/ml Article Title: Heavy chain-only antibodies with a stabilized human VH in transgenic chickens for therapeutic antibody discovery Article Snippet: .. Chicken primordial germ cells (PGCs) were derived and cultured as described, in KO-DMEM (ThermoFisher 0,829,018) containing 40% buffalo rat liver (BRL)-conditioned medium, 7.5% fetal calf serum (FCS) (Hyclone, SH30088.03), 2.5% chicken serum (ThermoFisher 16,110,082), 2 mm Glutamax (ThermoFisher 35,050–061), 1 mm pyruvate (ThermoFisher 11,360-070), 1X non-essential amino acids (ThermoFisher 11,140–050), 0.1 mm β-mercapto-ethanol (ThermoFisher 21,985–023), 6 ng/ml In Vitro:Article Title: Multiplex genome editing of mammalian cells for producing recombinant heparin. Article Snippet: Primary murine mast cells were generated by extracting bone marrow cells from the femurs of 5–8-week-old female mice and culturing the cells in RPMI 1640 medium (Gibco) supplemented with 10% inactivated FBS (ThermoFisher), 25 mM HEPES (pH 7.4), 4 mM Lglutamine, 0.1 mM nonessential amino acids, 1 mM sodium pyruvate, 50 μM β-mercaptoethanol, 100 IU/mL penicillin and 100 μg/ml streptomycin. .. Recombinant murine IL-3 (1 ng/mL; R&D Systems) and |